Staff Research Associate

We
are seeking a skilled Staff Research Associate with experience in a BSL2
laboratory. The successful candidate will be responsible for a variety of
tasks, including:

• Tissue Culture: Maintain various human and mouse cell lines.
• Assays: Perform cell proliferation, cytotoxicity, and apoptosis
  assays, as well as functional assays (cytokine assays) on human hematopoietic
  stem and cancer cells.
• Molecular Biology: Conduct DNA and RNA extraction, molecular
  cloning, PCR, and RT-PCR. Design primers for RT/PCR.
• Protein Analysis: Perform protein extraction, quantification, and
  Western Blots.
• Sample Processing: Process blood and tissue samples (mouse and
  human) and sort for selected cell populations.
• Collaboration: Work closely with other laboratory personnel on
  large-scale experiments. Assist in training undergraduates.
• Inventory Management: Order, stock, and maintain laboratory
  supplies.
• Animal Handling: Handle mice, including tumor cell injections,
  treatment administration, subcutaneous, intraperitoneal, and oral gavage, as
  well as euthanization and tissue collection for formalin fixation and paraffin
  embedding at the Translational Pathology Core Laboratory. Assist with humanized
  mouse experiments.
• Safety Compliance: Adhere to safety lab procedures.

If
you are looking to contribute to a dynamic research team, we encourage you to
apply!

Salary
offers are determined based on various factors including, but not limited to,
qualifications, experience, and equity. The full hourly salary range for this position
is $25.75 - $30.71.

Required:

• Bachelor's degree or
  higher in biology or related field. Working knowledge and experience with
  basic biology.
• Skill in using BSL2+
  laboratory practices and sterile techniques.
• Skill in safely handling
  mice.
• Skill in performing
  animal manipulations- various injections, oral gavage, euthanize, tissue
  harvesting.
• Skill in processing
  mouse tissues (blood, spleen, thymus, lung, liver, brain, bone marrow, and
  more) in a biosafety cabinet.
• Skill in preparing media
  and reagents used in tissue culture work.
• Skill in handling human
  cells and cell lines (adherent and suspension). Skill in counting cells,
  freezing/thawing cells, storing cells, passaging cell lines, maintaining
  primary cells.
• Skill in flow cytometry
  acquisition and analysis: MACSQuant and Fortessa flow cytometer use.
• Skill in performing
  DNA/RNA extractions, viral RNA, PCR, qRT-PCR.
• Willing to work long
  hours (planned or unplanned), overtime, or alternate hours (such as weekends,
  offset hours and late at night) as requested.
• Skill in performing a
  given protocol in a reproducible way. Skill in reading laboratory
  protocols and other scientific documents. Skill in troubleshooting and
  optimizing protocols as necessary, documenting any protocol deviations with
  thorough explanation of why deviation occurred.